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Anti-inflammatory effect of amitriptyline


Article published in Journal of Pharmacy & Pharmacognosy Research 5(3): 144-155, 2017. (PDF)

Original Article

In vitro and in vivo modulation of LPS and carrageenan-induced expression of inflammatory genes by amitriptyline

Laleh Rafiee1, Valiollah Hajhashemi2*, Shaghayegh Haghjooy Javanmard3

1Applied Physiology Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
2Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
3Applied Physiology Research Center, Isfahan Cardiovascular Research Institute and Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

*E-mail: vhajhashemi@gmail.com

Abstract

Context: Amitriptyline, a tricyclic antidepressant is used for the management of psychological disorders and various types of pain. In the previous work, it is founded that amitriptyline inhibited the migration of polymorphonuclear (PMN) into the site of inflammation.

Aims: To evaluate the effect of amitriptyline on the expression of some inflammatory mediators such as intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), cyclooxygenase 2 (COX2) and inducible nitric oxide synthase (iNOS).

Methods: An in vitro model system of LPS-stimulated human endothelial cells and U937 macrophages and also in vivo model of carrageenan-induced paw edema in rat were used. The expression of inflammatory mediator genes was determined by qRT-Real-time PCR. In endothelial cells, soluble forms of ICAM-1 and VCAM-1 were quantified by ELISA.

Results: The expression of ICAM-1, VCAM-1, COX2, iNOS, sICAM-1 and sVCAM-1 significantly decreased by amitriptyline. The finding of this study also confirmed that intraperitoneal (i.p.) injection of amitriptyline inhibited carrageenan-induced inflammation in rat paw edema.

Conclusions: The results of the present study provide further evidence for the anti-inflammatory effect of amitriptyline. This effect appears to be mediated by down-regulation of inflammatory genes.

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This entry was posted on 22 de April de 2017 by in Cellular Pharmacology, Inflammation, Molecular Pharmacology, Pharmacology, Preclinical Research.

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