Anticataract activity of ethanolic extract from Hippobroma longiflora (L.) G.Don leaves: Ex vivo investigation

Article published in J. Pharm. Pharmacogn. Res., vol. 11, no. 5, pp. 833-840, Sep-Oct 2023.

DOI: https://doi.org/10.56499/jppres23.1691_11.5.833

Original Article

Eva Imelda^1,2,3, Khairan Khairan⁴, Rodiah R. Lubis⁵, Pati Kemala¹, Utari Zulfiani^6,8, Suci Rahayu⁸, Ghazi M. Idroes⁷, Sarra M. Adev⁹, Nur B. Maulydia¹, Rinaldi Idroes^4,8*

¹Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.

²Department of Ophthalmology, General Hospital Dr. Zainoel Abidin, Banda Aceh 23126, Indonesia.

³Department of Ophthalmology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.

⁴Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.

⁵Department of Ophthalmology, Faculty of Medicine, Universitas Sumatera Utara, Medan 20222, Indonesia.

⁶Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Surabaya, Surabaya 60111, Indonesia.

⁷Department of Occupational Health and Safety, Faculty of Health Sciences, Abulyatama University, Banda Aceh 23372, Indonesia.

⁸Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.

9Medical Programme, Faculty of Medicine Universitas Brawijaya, Malang 65145, Indonesia.

*E-mail: rinaldi.idroes@usk.ac.id

Abstract

Context: In Aceh, Hippobroma longiflora, known as kitolodby the locals, is believed to possess medicinal benefits such as anticataract.

Aims: To investigate the anticataract activity of ethanolic extract from H. longiflora leaves ex vivo.

Methods: The dried fine powder of H. longiflora was macerated using ethanol at room temperature, and the concentrated extract was prepared in concentrations ranging from 100 to 300 μg/mL. The cataract model was established by using goat lens induced with H₂O₂ and later exposed to the extract for treatment. Anti-cataract activity was assessed by observing the change in lens opacity. Further, malondialdehyde levels and activities of endogenous antioxidants (superoxide dismutase, glutathione peroxidase, and catalase).

Results: The apparent cataract opacity reduced after the exposure of the extract was observed macroscopically. The cataract eyes experienced a significant increase (p<0.05) in malondialdehyde level and a significant reduction (p<0.05) in the activities of superoxide dismutase, glutathione peroxidase, and catalase. The pathologic conditions could be reversed by introducing the extract with a concentration as low as 100 μg/mL (p<0.05).

Conclusions: The ethanolic extract of H. longiflora leaf has a potential anticataract effect by ameliorating oxidative stress-related biocompounds.